Glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter

نویسندگان

  • Tanja Hanke
  • Katharina Nöh
  • Stephan Noack
  • Tino Polen
  • Stephanie Bringer
  • Wolfgang Wiechert
  • Michael Bott
چکیده

Glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter 1 oxydans 621H: a combined fluxomics and transcriptomics analysis 2 3 Tanja Hanke, Katharina Nöh,* Stephan Noack, Tino Polen, Stephanie Bringer, Hermann 4 Sahm, Wolfgang Wiechert, Michael Bott* 5 Institut für Biound Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum Jülich, 6 D-52425 Jülich, Germany 7 8 9

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Glucose catabolism via a partially cyclic pentose phosphate pathway in

Glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter 1 oxydans 621H: a combined fluxomics and transcriptomics analysis 2 3

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Glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter 1 oxydans 621 H : a combined fluxomics and transcriptomics analysis

Glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter 1 oxydans 621H: a combined fluxomics and transcriptomics analysis 2 3 Tanja Hanke, Katharina Nöh,* Stephan Noack, Tino Polen, Stephanie Bringer, Hermann 4 Sahm, Wolfgang Wiechert, Michael Bott* 5 Institut für Biound Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum Jülich, 6 D-52425 Jülich, Germany 7 8 9

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Combined fluxomics and transcriptomics analysis of glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter oxydans 621H.

In this study, the distribution and regulation of periplasmic and cytoplasmic carbon fluxes in Gluconobacter oxydans 621H with glucose were studied by (13)C-based metabolic flux analysis ((13)C-MFA) in combination with transcriptomics and enzyme assays. For (13)C-MFA, cells were cultivated with specifically (13)C-labeled glucose, and intracellular metabolites were analyzed for their labeling pa...

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13C Tracers for Glucose Degrading Pathway Discrimination in Gluconobacter oxydans 621H

Gluconobacter oxydans 621H is used as an industrial production organism due to its exceptional ability to incompletely oxidize a great variety of carbohydrates in the periplasm. With glucose as the carbon source, up to 90% of the initial concentration is oxidized periplasmatically to gluconate and ketogluconates. Growth on glucose is biphasic and intracellular sugar catabolism proceeds via the ...

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Role of Hexose Monophosphate Pathway in Tomato Catabolism.

The operation of the hexose monophosphate pathway (HMP) in plants has been the object of extensive studies in recent years (1, 2, 4). In previous papers (3, 9) it has been demonstrated that in intact tomato fruit, glucose is catabolized mainly by way of the Embden-Meyerhof-Parnas pathway (EMP) and to a limited extent via the HMP pathway. However, the catabolic fate of the pentose phosphate, der...

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تاریخ انتشار 2013